《植物生理学报》 2024, 60(8): 1221-1228

薯蓣皂苷元生物合成途径及其关键基因研究进展

摘　要：

李文砚1, 黄静丽1, 李长洲1, 黄金梅1, 肖冬1,2,3, 詹洁1,2,3, 王爱勤1,2,3, 张平刚4, 何龙飞1,2,3,4,*

1广西大学农学院, 南宁530004

2广西农业环境与农产品安全重点实验室, 南宁530004

3广西高校作物栽培与生理重点实验室, 南宁530004

4广西大学农牧产业发展研究院, 南宁530004

*通信作者(lfhe@gxu.edu.cn)

摘要: 薯蓣皂苷元属甾体皂苷类化合物, 是薯蓣属植物中代表性的药用成分, 具多重药理学活性, 也是300

多种甾体激素类药物的重要前体物质。本文简要概述了薯蓣皂苷元在植物中的分布, 薯蓣皂苷元生物合

成过程及关键酶基因角鲨烯环氧酶、环阿屯醇合酶、细胞色素P450酶和尿苷二磷酸糖基转移酶的研究

进展, 并解读它们参与薯蓣皂苷元合成途径的催化机制, 以便为提高植物中薯蓣皂苷元含量, 促进薯蓣遗

传育种与栽培提供参考。

关键词: 薯蓣皂苷元; 甾体皂苷; 生物合成; 次生代谢物

Advances on diosgenin biosynthesis pathways and their key genes

LI Wenyan1, HUANG Jingli1, LI Changzhou1, HUANG Jinmei1, XIAO Dong1,2,3, ZHAN Jie1,2,3,

WANG Aiqin1,2,3, ZHANG Pinggang4, HE Longfei1,2,3,4,*

1College of Agronomy, Guangxi University, Nanning 530004, China

2Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning 530004, China

3Key Laboratory of Crop Cultivation and Physiology, Guangxi University, Education Department of Guangxi Zhuang

Autonomous Region, Nanning 530004, China

4Agricultural and Animal Husbandry Industry Development Research Institute, Guangxi University, Nanning 530004, China

*Corresponding author (lfhe@gxu.edu.cn)

Abstract: Diosgenin, a steroid saponin compound, is a representative medicinal component in Dioscorea.

It plays a direct role in multiple pharmacological activities. Diosgenin is also an important precursor sub-

stance to produce more than 300 steroid hormones. In this paper, the research progresses on the distribu-

tion, biosynthesis process, function, and its catalytic mechanism of diosgenin biosynthesis key enzyme

genes are reviewed, including squalene epoxidase, cycloartenol synthase, cytochrome P450 enzyme and

uridine diphosphate glycosyltransferase etc. It provides the reference for improving the content of dios-

genin in plants and promoting genetic breeding and cultivation of Dioscorea.

Key words: diosgenin; steroid saponin; biosynthesis; secondary metabolite

收稿 2023-08-22 修定 2024-06-10

资助 财政部和农业农村部: 国家现代农业产业技术体系资助(CARS-21)、国家自然科学基金(81860670)、广西自然科学基金重点项目

(2018GXNSFDA281029)和国家现代农业产业技术体系-广西创新团队首席专家项目(nycytxgxcxtd-11-01)。